Anti-Histone H2B (acetyl Lys 15) Antibody (A320119)

Anti-Histone H2B (acetyl Lys 15) Antibody

Rabbit polyclonal antibody to Histone H2B (acetyl Lys 15).

This antibody recognises Histone H2B when acetylated at lysine 15.Histone H2B is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). Histone H2B aids the regulation of chromatin structure and function via post-translational modifications of specialized histone variants. Lysine residues within the N-terminal tail protruding from the histone core of the nucleosome are acetylated and deacetylated as part of gene regulation. Hyperacetylation of histone tails makes chromatin more accessible to DNA-binding proteins by weakening histone-DNA and nucleosome-nucleosome interactions. Acetylation of a specific lysine residues on histones allows binding to bromine-containing domains of particular transcription and chromatin regulatory proteins. Wide species cross-reactivity is expected from Rabbit anti Human histone H2B (Ac15) antibody based on sequence.

WB: 1:500 - 1:1,000

Human

A peptide containing acetylated lysine 15 of human histone H2B.

Rabbit

Polyclonal

IgG

Lot Specific

Liquid

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rabbit anti Human histone H2B (Ac15) antibody recognizes histone H2B when acetylated at lysine 15.Histone H2B is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). Histone H2B aids the regulation of chromatin structure and function via post-translational modifications of specialized histone variants (Molden et al. 2015). Lysine residues within the N-terminal tail protruding from the histone core of the nucleosome are acetylated and deacetylated as part of gene regulation. Hyperacetylation of histone tails makes chromatin more accessible to DNA-binding proteins by weakening histone-DNA and nucleosome-nucleosome interactions. Acetylation of a specific lysine residues on histones allows binding to bromine-containing domains of particular transcription and chromatin regulatory proteins (Kadiyala et al. 2012).Wide species cross-reactivity is expected from Rabbit anti Human histone H2B (Ac15) antibody based on sequence.

H2B K, H2B type 12, H2B-clustered histone 12 like, H2B-clustered histone 17, H2B-clustered histone 18, H2B-clustered histone 21, H2B-clustered histone 26, H2B-clustered histone 3, H2B-clustered histone 5, H2B-clustered histone 9, H2B.S histone 1, H2B.U histone 1, H2B/a, H2B/b, H2B/c, H2B/d, H2B/e, H2B/f, H2B/g, H2B/h, H2B/j, H2B/k, H2B/l, H2B/n, H2B/q, H2B/s, H2BC12, H2BC12L, H2BC13, H2BC14, H2BC15, H2BC17, H2BC18, H2BC21, H2BC26, H2BC3, H2BC4, H2BC5, H2BC9, H2BFB, H2BFC, H2BFD, H2BFE, H2BFF, H2BFH, H2BFJ, H2BFL, H2BFN, H2BFQ, H2BFS, H2BFT, H2BS1, H2BU1, HIRA-interacting protein 1, HIRA-interacting protein 2, HIRIP1, HIRIP2, HIST1H2BB, HIST1H2BC, HIST1H2BD, HIST1H2BH, HIST1H2BK, HIST1H2BL, HIST1H2BM, HIST1H2BN, HIST1H2BO, HIST2H2BE, HIST2H2BF, HIST3H2BB, Histone H2B type 1-B, Histone H2B type 1-C/E/F/G/I, Histone H2B type 1-D, Histone H2B type 1-H, Histone H2B type 1-K, Histone H2B type 1-L, Histone H2B type 1-M, Histone H2B type 1-N, Histone H2B type 1-O, Histone H2B type 2-E, Histone H2B type 2-F, Histone H2B type 3-B, Histone H2B type F-S, Histone H2B-GL105, Histone H2B.1, Histone H2B.1 A, Histone H2B.1 B, Histone H2B.2, Histone H2B.a, Histone H2B.b, Histone H2B.c, Histone H2B.d, Histone H2B.e, Histone H2B.f, Histone H2B.g, Histone H2B.h, Histone H2B.j, Histone H2B.k, Histone H2B.l, Histone H2B.n, Histone H2B.q, Histone H2B.s