Anti-Histone H2B (mono methyl Lys5) Antibody (A320120)

Anti-Histone H2B (mono methyl Lys5) Antibody

Rabbit polyclonal antibody to Histone H2B (mono methyl Lys5).

This antibody recognises Histone H2B when mono-methylated at lysine 5.Histone H2B is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). The presence of post-translational modifications and specialized variants of histone H2B helps the regulation of chromatin structure and function. It has been suggested that changes in methylation of histone H2B may be involved in heat-shock mechanisms. Mono-methylation of lysine 5 is thought to occur at active promoters downstream of the transcription start sight. Wide species cross-reactivity is expected from Rabbit anti Human mono-methyl-histone H2B (Lys5) antibody based on sequence.

WB: 1:500 - 1:1,000

Human, Broad

Synthetic peptide of mono-methyl lysine 5 of human histone H2B.

Rabbit

Polyclonal

IgG

Lot Specific

Liquid

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rabbit anti Human mono-methyl-histone H2B (Lys5) antibody recognizes histone H2B when mono-methylated at lysine 5.Histone H2B is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). The presence of post-translational modifications and specialized variants of histone H2B helps the regulation of chromatin structure and function (Molden et al. 2015). It has been suggested that changes in methylation of histone H2B may be involved in heat-shock mechanisms. Mono-methylation of lysine 5 is thought to occur at active promoters downstream of the transcription start sight (Wozniak and Strahl, 2014).Wide species cross-reactivity is expected from Rabbit anti Human mono-methyl-histone H2B (Lys5) antibody based on sequence.

H2B K, H2B type 12, H2B-clustered histone 12 like, H2B-clustered histone 17, H2B-clustered histone 18, H2B-clustered histone 21, H2B-clustered histone 26, H2B-clustered histone 3, H2B-clustered histone 5, H2B-clustered histone 9, H2B.S histone 1, H2B.U histone 1, H2B/a, H2B/b, H2B/c, H2B/d, H2B/e, H2B/f, H2B/g, H2B/h, H2B/j, H2B/k, H2B/l, H2B/n, H2B/q, H2B/s, H2BC12, H2BC12L, H2BC13, H2BC14, H2BC15, H2BC17, H2BC18, H2BC21, H2BC26, H2BC3, H2BC4, H2BC5, H2BC9, H2BFB, H2BFC, H2BFD, H2BFE, H2BFF, H2BFH, H2BFJ, H2BFL, H2BFN, H2BFQ, H2BFS, H2BFT, H2BS1, H2BU1, HIRA-interacting protein 1, HIRA-interacting protein 2, HIRIP1, HIRIP2, HIST1H2BB, HIST1H2BC, HIST1H2BD, HIST1H2BH, HIST1H2BK, HIST1H2BL, HIST1H2BM, HIST1H2BN, HIST1H2BO, HIST2H2BE, HIST2H2BF, HIST3H2BB, Histone H2B type 1-B, Histone H2B type 1-C/E/F/G/I, Histone H2B type 1-D, Histone H2B type 1-H, Histone H2B type 1-K, Histone H2B type 1-L, Histone H2B type 1-M, Histone H2B type 1-N, Histone H2B type 1-O, Histone H2B type 2-E, Histone H2B type 2-F, Histone H2B type 3-B, Histone H2B type F-S, Histone H2B-GL105, Histone H2B.1, Histone H2B.1 A, Histone H2B.1 B, Histone H2B.2, Histone H2B.a, Histone H2B.b, Histone H2B.c, Histone H2B.d, Histone H2B.e, Histone H2B.f, Histone H2B.g, Histone H2B.h, Histone H2B.j, Histone H2B.k, Histone H2B.l, Histone H2B.n, Histone H2B.q, Histone H2B.s