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Recombinant Anti-Histone H3 (crotonyl Lys9) Antibody [RM339] (A121227)

Dot Blot - Anti-Histone H3 (crotonyl Lys9) Antibody [RM339] (A121415) - Antibodies.com Western Blot - Anti-Histone H3 (crotonyl Lys9) Antibody [RM339] (A121227) - Antibodies.com
Recombinant Antibody
$585
Product Datasheet

Recombinant rabbit monoclonal [RM339] antibody to Histone H3 (crotonyl Lys9) for WB and Dot.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-8 business days.

  • Specifications

Name
Anti-Histone H3 (crotonyl Lys9) Antibody [RM339]
Description
Recombinant rabbit monoclonal [RM339] antibody to Histone H3 (crotonyl Lys9).
Specificity
This antibody recognizes Histone H3 crotonylated at Lysine 9.
Applications
WB, Dot
Dilutions
WB: 1-5 µg/ml, DB: 0.5-2 µg/ml, Multiplex Protein Detection: 0.05-0.5 µg/ml
Reactivity
All Vertebrates
Cross Reactivity
There is no cross reactivity with acetylated or butyrylated Lysine 9 or with other crotonylated Lysines in Histone H3.
Immunogen
Synthetic peptide corresponding to Histone H3 (crotonyl Lys9).
Host
Rabbit
Clonality
Monoclonal
Clone ID
RM339
Isotype
IgG
Conjugate

Unconjugated

Purification
Protein A chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
H3-3A, H3-5, H3-clustered histone 13, H3-clustered histone 14, H3-clustered histone 15, H3.3A, H3C1, H3C15, H3F3, H3F3A, H3F3C, H3FA, HIST1H3A, HIST2H3A, Histone H3.1, Histone H3.2, Histone H3.3, Histone H3.3C, Histone H3.5, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/m, Histone H3/o
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Dot Blot - Anti-Histone H3 (crotonyl Lys9) Antibody [RM339] (A121415) - Antibodies.comEnlarge Image

Dot Blot - Anti-Histone H3 (crotonyl Lys9) Antibody [RM339] (A121227)

A peptide dot blot showing that Anti-Histone H3 (crotonyl Lys9) Antibody [RM339] reacts specifically to Histone H3 crotonylated at Lysine 9 (H3K9-Crotonyl), and it’s cross-reactivity with different peptides.
Western Blot - Anti-Histone H3 (crotonyl Lys9) Antibody [RM339] (A121227) - Antibodies.comEnlarge Image

Western Blot - Anti-Histone H3 (crotonyl Lys9) Antibody [RM339] (A121227)

Western blot using Anti-Histone H3 (crotonyl Lys9) Antibody [RM339] against H3K9cr [crotonyl Histone H3 (Lys9)]. Anti-Histone H3 Antibody and Anti-GAPDH Antibody were used as controls. A crotonylation inducing metabolite was used to increase the H3K9cr signal.
Publishing research using Recombinant Anti-Histone H3 (crotonyl Lys9) Antibody [RM339] (A121227)? Please let us know so that we can list the citation on this page.

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