The antibody detects endogenous level of total HSP27 protein.
Applications
WB, IHC
Dilutions
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100
Reactivity
Human
Immunogen
Peptide sequence around aa.76~80 (A-L-S-R-Q) derived from Human HSP27.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Concentration
1 mg/ml
Predicted MW
27kd
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Synonyms
28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, Heat shock protein beta-1, Heat shock protein family B member 1, HSP 27, HSP28, HspB1, SRP27, Stress-responsive protein 27
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27 (Ab-78) Antibody #21239 (left) or the same antibody preincubated with blocking peptide (right).
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