This antibody does not cross-react with HDJ2 or YDJ1.
Immunogen
Recombinant Human Protein HSP40 (Hdj1).
Host
Mouse
Clonality
Monoclonal
Clone ID
3B9.E6
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein G purification.
Concentration
1 mg/ml
Molecular Weight
~40 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.2, with 50% Glycerol and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
DnaJ homolog subfamily B member 1, DnaJ protein homolog 1, DNAJ1, DNAJB1, hDj-1, HDJ1, Heat shock 40 kDa protein 1, Heat shock protein 40, HSPF1, Human DnaJ protein 1
Western Blot - Anti-HSP40 Antibody [3B9.E6] (A304769)
Western blot analysis of human Cell lysates showing detection of Hsp40 protein using Anti-HSP40 Antibody [3B9.E6] (A304769) at 1:1,000 for 2 hours at room temperature. Load: 15 µg. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Sheep Anti-Mouse IgG: HRP for 1 hour at room temperature.
Immunocytochemistry/Immunofluorescence analysis of human HaCaT cells, fixed in cold 100% methanol for 10 minutes at -20°C, using Anti-HSP40 Antibody [3B9.E6] (A304769), at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature.
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