Recombinant purified full-length human HSP40 (no tags).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Molecular Weight
~40 kDa
Purity
Whole antiserum.
Product Form
Liquid
Formulation
Supplied as an aliquot of antiserum.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
DnaJ homolog subfamily B member 1, DnaJ protein homolog 1, DNAJ1, DNAJB1, hDj-1, HDJ1, Heat shock 40 kDa protein 1, Heat shock protein 40, HSPF1, Human DnaJ protein 1
Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-HSP40 Antibody (A304850), at 1:100 for 12 hours at 4°C. The secondary antibody used was APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Cytoplasm. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-Hsp40 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
Western blot analysis of human Cervical cancer cell line (HeLa) lysate showing detection of HSP40 protein using Anti-HSP40 Antibody (A304850) at 1:1,000.
Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-HSP40 Antibody (A304850), at 1:100 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Cytoplasm. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-Hsp40 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
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