Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
DnaJ homolog subfamily B member 1, DnaJ protein homolog 1, DNAJ1, DNAJB1, hDj-1, HDJ1, Heat shock 40 kDa protein 1, Heat shock protein 40, HSPF1, Human DnaJ protein 1
Western blot analysis of extracts of various cell lines, using Anti-Hsp40 Antibody (A14812) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 15s.
Western blot analysis of extracts from normal (control) and DNAJB1 knockout (KO) 293T cells, using Anti-Hsp40 Antibody (A14812) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.