Anti-HSP60 Antibody [1C7] (A85439)

Anti-HSP60 Antibody [1C7]

Mouse monoclonal [1C7] antibody to HSP60.

WB, ICC/IF, IHC

WB: 1:10,000, ICC/IF: 1:5,000, IHC: 1:5,000

Human, Rat, Mouse

Spontaneous autoantibody which binds LNERLAKLSDGVAVLKVGGT, amino acids 390-409 of the human sequence.

Mouse

Monoclonal

1C7

IgG1

Immunogen affinity purification.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 50% Glycerol and 5mM Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

This antibody was discovered during screens for antibodies produced by hybridomas from a mouse injected with an unrelated protein. We noted beautiful staining of mitochondria and clean staining of a single band of 60 kDa on western blots, suggesting HSP60 as an obvious candidate. We made recombinant full-length human HSP60 which this antibody bound to strongly and specifically. The mouse had likely developed autoantibodies to HSP60 since this molecule appears to be unusually immunogenic, frequently generating autoantibodies in humans and other species.

60 kDa chaperonin, 60 kDa heat shock protein, mitochondrial, Chaperonin 60, CPN60, Heat shock protein 60, Heat shock protein family D member 1, HSP-60, HSPD1, HuCHA60