Recombinant humanized monoclonal antibody to CD98.
Applications
ELISA, Flow Cytometry
Dilutions
ELISA 1:5,000-1:10,000, Flow Cytometry: 1:100
Reactivity
Human
Host
Humanized
Clonality
Monoclonal
Isotype
IgG1 (K97R)
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Synonyms
4F2 cell-surface antigen heavy chain, 4F2 heavy chain antigen, 4F2hc, Amino acid transporter heavy chain SLC3A2, Lymphocyte activation antigen 4F2 large subunit, MDU1, SLC3A2, Solute carrier family 3 member 2
Immobilised Recombinant Human CD98 Protein (6xHis Tag) (A317671) at 2 µg/ml (100 µl/well) can bind HuM23 Biosimilar - Anti-CD98 Antibody in a linear range of 0.64–80 ng/ml.
CD98 expresion in Expi293 cells analyzed by flow cytometry. Staining was performed with HuM23 Biosimilar - Anti-CD98 Antibody (Blue histogram) or isotype control monoclonal antibody (Red histogram) on Expi293 cells.
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