This antibody recognises non-classical MHC class I chain A (MICA) and non-classical MHC class I chain MICB (MICB). MICA and MICB are stress inducible antigens, which are closely related and appear functionally indistinguishable. MICA and MICB are ligands for NKG2D, an activating receptor on most natural killer (NK) cells, CD8 T cells and gamma delta T cells. MICA is principally expressed on intestinal epithelium, and several epithelial tumors. Expression may be induced to high surface levels by heat shock, oxidative stress, and virus infection. Clone 6D4 is reported to inhibit the cytotoxicity of NK cells stimulated by IFN alpha-treated dendritic cells.
Applications
IP, Flow Cytometry, IHC-Fr
Dilutions
Flow Cytometry: 1:50 - 1:200, IHC-Fr: 1:100, Use 10µl of the suggested working dilution to label 1x106 cells in 100µl
Reactivity
Human
Immunogen
MICA transfected C1R cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
6D4
Isotype
IgG2a
Conjugate
Unconjugated
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Human MICA/Micb antibody, clone 6D4 recognizes non-classical MHC class I chain A (MICA) and non-classical MHC class I chain MICB (MICB). MICA and MICB are stress inducible antigens, which are closely related and appear functionally indistinguishable. MICA and MICB are ligands for NKG2D, an activating receptor on most natural killer (NK) cells, CD8 T cells and gamma delta T cells. MICA is principally expressed on intestinal epithelium, and several epithelial tumors. Expression may be induced to high surface levels by heat shock, oxidative stress, and virus infection. Clone 6D4 is reported to inhibit the cytotoxicity of NK cells stimulated by IFN alpha-treated dendritic cells (Jinushi et al. 2003).
Synonyms
MHC class I chain-related protein A, MHC class I chain-related protein B, MHC class I polypeptide related sequence A, MHC class I polypeptide related sequence B, MHC class I polypeptide-related sequence A, MIC-A, micA, MICA_HUMAN, MICB
Recombinant human monoclonal antibody to MICA + MICB for use as a research grade Anti-Human MICA + B for ELISA, Flow Cytometry, Functional Studies and in vivo Research.
Recombinant human monoclonal antibody to MICA + MICB for use as a research grade isuventatug biosimi for ELISA, Flow Cytometry, Functional Studies and in vivo Research.
Recombinant human monoclonal antibody to MICA + MICB for use as a research grade 36 NFG 236A biosimi for ELISA, Flow Cytometry, Functional Studies and in vivo Research.
Recombinant mouse monoclonal antibody to MICA + MICB for use as a research grade PDI-01 biosimilar for ELISA, Flow Cytometry, Functional Studies and in vivo Research.
Recombinant human monoclonal antibody to MICA + MICB for use as a research grade IPH4301 biosimilar for ELISA, Flow Cytometry, Functional Studies and in vivo Research.
Recombinant human monoclonal antibody to MICA + MICB for use as a research grade Anti-Human MICA + B for ELISA, Flow Cytometry, Functional Studies and in vivo Research.
Recombinant human monoclonal antibody to MICA + MICB for use as a research grade isuventatug biosimi for ELISA, Flow Cytometry, Functional Studies and in vivo Research.
Recombinant human monoclonal antibody to MICA + MICB for use as a research grade 36 NFG 236A biosimi for ELISA, Flow Cytometry, Functional Studies and in vivo Research.
Recombinant mouse monoclonal antibody to MICA + MICB for use as a research grade PDI-01 biosimilar for ELISA, Flow Cytometry, Functional Studies and in vivo Research.
Recombinant human monoclonal antibody to MICA + MICB for use as a research grade IPH4301 biosimilar for ELISA, Flow Cytometry, Functional Studies and in vivo Research.
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