Anti-MICA + MICB Antibody [6D4] - BSA and Azide free (A279478)

Anti-MICA + MICB Antibody [6D4] - BSA and Azide free

Mouse monoclonal [6D4] antibody to MICA + MICB.

This antibody recognises non-classical MHC class I chain A (MICA) and non-classical MHC class I chain MICB (MICB). MICA and MICB are stress inducible antigens, which are closely related and appear functionally indistinguishable. MICA and MICB are ligands for NKG2D, an activating receptor on most natural killer (NK) cells, CD8 T cells and gamma delta T cells. MICA is principally expressed on intestinal epithelium, and several epithelial tumors. Expression may be induced to high surface levels by heat shock, oxidative stress, and virus infection. Clone 6D4 is reported to inhibit the cytotoxicity of NK cells stimulated by IFN alpha-treated dendritic cells.

IHC-Fr, Flow Cytometry, IP, Functional Assays

Flow Cytometry: 1:50 - 1:200, IHC-Fr: 1:100, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl

Human

MICA transfected C1R cells.

Mouse

Monoclonal

6D4

IgG2a

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline.

Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.

Mouse anti Human MICA/Micb antibody, clone 6D4 recognizes non-classical MHC class I chain A (MICA) and non-classical MHC class I chain MICB (MICB). MICA and MICB are stress inducible antigens, which are closely related and appear functionally indistinguishable. MICA and MICB are ligands for NKG2D, an activating receptor on most natural killer (NK) cells, CD8 T cells and gamma delta T cells. MICA is principally expressed on intestinal epithelium, and several epithelial tumors. Expression may be induced to high surface levels by heat shock, oxidative stress, and virus infection. Clone 6D4 is reported to inhibit the cytotoxicity of NK cells stimulated by IFN alpha-treated dendritic cells (Jinushi et al. 2003).

MHC class I chain-related protein A, MHC class I chain-related protein B, MHC class I polypeptide related sequence A, MHC class I polypeptide related sequence B, MHC class I polypeptide-related sequence A, MIC-A, micA, MICA_HUMAN, MICB