Rabbit polyclonal antibody to Mst1 (Phospho-Thr183)
Specificity
The antibody detects endogenous levels of Mst1 only when phosphorylated at threonine 183.
Applications
WB, IHC
Dilutions
Western blotting: 1:500~1:3000 Immunohistochemistry: 1:50~1:100
Reactivity
Human, Mouse
Immunogen
Peptide sequence around phosphorylation site of threonine 183 (R-N-T(p)-V-I) derived from Human Mst1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from 293 cells, treated with H2O2 (100uM, 15mins), using Mst1 (Phospho-Thr183) antibody #12144. The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Mst1 (Phospho-Thr183) antibody #12144. The picture on the right is treated with the synthesized peptide.
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