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Anti-NPM (phospho Thr199) Antibody (A93780)

Immunofluorescence - Anti-NPM (phospho Thr199) Antibody (A0694) - Antibodies.com Immunohistochemistry - Anti-NPM (phospho Thr199) Antibody (A0694) - Antibodies.com Western Blot - Anti-NPM (phospho Thr199) Antibody (A0694) - Antibodies.com
$190
Product Datasheet

Rabbit polyclonal antibody to NPM (phospho Thr199) for WB, IHC, IF and ELISA.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 6-8 business days.

  • Specifications

Name
Anti-NPM (phospho Thr199) Antibody
Description
Rabbit polyclonal antibody to NPM (phospho Thr199).
Specificity
This antibody detects endogenous levels of NPM only when phosphorylated at Thr199.
Applications
WB, IHC, IF, ELISA
Dilutions
WB: 1:500-1:1000, IHC: 1:50-1:100, IF: 1:100-1:500, ELISA: 1:60000
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide derived from human NPM around the phosphorylation site of Thr199 (amino acids 171-220).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
32kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
NPM, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Numatrin
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Immunofluorescence - Anti-NPM (phospho Thr199) Antibody (A0694) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-NPM (phospho Thr199) Antibody (A93780)

Immunofluorescence analysis of HeLa cells, treated with EGF 200nM 5', using Anti-NPM (phospho Thr199) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Immunohistochemistry - Anti-NPM (phospho Thr199) Antibody (A0694) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-NPM (phospho Thr199) Antibody (A93780)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Anti-NPM (phospho Thr199) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Western Blot - Anti-NPM (phospho Thr199) Antibody (A0694) - Antibodies.comEnlarge Image

Western Blot - Anti-NPM (phospho Thr199) Antibody (A93780)

Western blot analysis of lysates from HepG2 cells using Anti-NPM (phospho Thr199) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Publishing research using Anti-NPM (phospho Thr199) Antibody (A93780)? Please let us know so that we can list the citation on this page.

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Proteins predicted to interact with NPM1

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.7% Relevancy Score
99.4% Relevancy Score
99.2% Relevancy Score
98.3% Relevancy Score
97.6% Relevancy Score
97.5% Relevancy Score