Anti-NPM (phospho Thr234) Antibody (A94273)

$190

Rabbit polyclonal antibody to NPM (phospho Thr234) for WB and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 6-8 business days.

Specifications

Name

Anti-NPM (phospho Thr234) Antibody

Description

Rabbit polyclonal antibody to NPM (phospho Thr234).

Specificity

This antibody detects endogenous levels of NPM only when phosphorylated at Thr234.

Applications

WB, ELISA

Dilutions

WB: 1:500-1:1000, ELISA: 1:1000

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide derived from human NPM around the phosphorylation site of Thr234 (amino acids 201-250).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.

Molecular Weight

32kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

NPM, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Numatrin

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

Western Blot - Anti-NPM (phospho Thr234) Antibody (A94273)

Western blot analysis of lysates from HeLa cells treated with nocodazole 1µg/ml 18h using Anti-NPM (phospho Thr234) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

Immunohistochemistry - Anti-NPM (phospho Thr234) Antibody (A94273)

Immunohistochemical analysis of paraffin-embedded human brain using Anti-NPM (phospho Thr234) Antibody 1:100 (4°C overnight). The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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