Anti-NRF1 Antibody (A14829)

Name: Anti-NRF1 Antibody
Brand: Antibodies.com
SKU: A14829
Price: 270.0 GBP
Availability: InStock

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$355

Rabbit polyclonal antibody to NRF1 for WB, IP and ChIP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-NRF1 Antibody

Description

Rabbit polyclonal antibody to NRF1.

Applications

WB, IP, ChIP

Dilutions

WB: 1:500-1:1,000, ChIP: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 240-449 of human NRF1 (NP_001035199.1).

Sequence

WANVRSDVRTEEQKQRVSWTQALRTIVKNCYKQHGREDLLYAFEDQQTQTQATATHSIAHLVPSQTVVQTFSNPDGTVSLIQVGTGATVATLADASELPTTVTVAQVNYSAVADGEVEQNWATLQGGEMTIQTTQASEATQAVASLAEAAVAASQEMQQGATVTMALNSEAAAHAVATLAEATLQGGGQIVLSGETAAAVGALTGVQDAN

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

70 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Alpha palindromic-binding protein, Alpha-pal, NRF-1, Nuclear respiratory factor 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-NRF1 Antibody (A14829)

Western blot analysis of extracts of various cell lines, using Anti-NRF1 Antibody (A14829) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Chromatin Immunoprecipitation - Anti-NRF1 Antibody (A14829)

Chromatin immunoprecipitation (ChIP) analysis of extracts of HepG2 cells, using Anti-NRF1 Antibody (A14829) and Rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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