Anti-NRF1 Antibody [ARC0768] (A306763)

Name: Anti-NRF1 Antibody [ARC0768]
Brand: Antibodies.com
SKU: A306763
Price: 325.0 GBP
Availability: InStock

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$485

Rabbit monoclonal [ARC0768] antibody to NRF1 for WB, IHC, IP and ChIP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-NRF1 Antibody [ARC0768]

Description

Rabbit monoclonal [ARC0768] antibody to NRF1.

Applications

WB, IHC, IP, ChIP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, IP: 1:500-1:1,000, ChIP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 300-400 of human NRF1 (Q16656).

Sequence

LVPSQTVVQTFSNPDGTVSLIQVGTGATVATLADASELPTTVTVAQVNYSAVADGEVEQNWATLQGGEMTIQTTQASEATQAVASLAEAAVAASQEMQQGA

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0768

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

68 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Alpha palindromic-binding protein, Alpha-pal, NRF-1, Nuclear respiratory factor 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-NRF1 Antibody [ARC0768] (A306763)

Western blot analysis of extracts of various cell lines, using Anti-NRF1 Antibody [ARC0768] (A306763) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Western Blot - Anti-NRF1 Antibody [ARC0768] (A306763)

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Immunohistochemistry - Anti-NRF1 Antibody [ARC0768] (A306763)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-NRF1 Antibody [ARC0768] (A306763) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Western Blot - Anti-NRF1 Antibody [ARC0768] (A306763)

Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-NRF1 Antibody [ARC0768] (A306763). This Western blot was performed on the immunoprecipitate using Anti-NRF1 Antibody [ARC0768] (A306763) at a dilution of 1:1000.

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