Anti-Osteopontin Antibody (A320055)

Anti-Osteopontin Antibody

Goat polyclonal antibody to Osteopontin.

This antibody recognises an epitope within the C-terminal (CT) region of osteopontin, an acid-rich, multidomain, phosphorylated ~60 kDa glycoprotein. Osteopontin is a secreted chemokine-like protein and member of the SIBLING family, and is synthesised by many cell types.Osteopontin is involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10. It is essential in the pathway that leads to type I immunity. Osteoponitn is also thought to be important to cell-matrix interaction, and can bind fibronectin, collagen and calcium.Osteopontin has been suggested to have a role in atherosclerosis, bone remodelling, angiogenesis, wound healing and tissue injuries.

WB, ELISA

ELISA: 1:64,000, WB: 1 - 3.0 µg/ml

Human, Dog, Rat, Mouse

A peptide sequence of C-DPKSKEEDKHLK from the C-terminus of osteopontin.

C-DPKSKEEDKHLK

Goat

Polyclonal

IgG

Approximately 60 kDa in lysates of human thymus, tonsil and spleen. Approximate 30 kDa band observed in lysates of cell lines Jurkat and MOLT4. This band corresponds to the cleavage product from the 60 kDa glcoprotein.

Liquid

Supplied in TRIS Buffered Saline, 0.5% BSA and 0.02% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Goat anti Human Osteopontin antibody recognises an epitope within the C-terminal (CT) region of osteopontin, an acid-rich, multidomain, phosphorylated ~60 kDa glycoprotein. Osteopontin is a secreted chemokine-like protein and member of the SIBLING family, and is synthesised by many cell types.Osteopontin is involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10. It is essential in the pathway that leads to type I immunity. Osteoponitn is also thought to be important to cell-matrix interaction, and can bind fibronectin, collagen and calcium.Osteopontin has been suggested to have a role in atherosclerosis, bone remodelling, angiogenesis, wound healing and tissue injuries.

BNSP, Bone sialoprotein 1, Nephropontin, OPN, Secreted phosphoprotein 1, SPP-1, SPP1, Urinary stone protein, Uropontin