Peptide sequence around phosphorylation site of threonine 423 / 402 / 421 (R-S-T(p)-M-V) derived from Human PAK1 / PAK2 / PAK3.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Predicted MW
61-67 (PAK2) 68-74 (PAK1 / 3)kd
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Synonyms
Alpha-PAK, p21-activated kinase 1, p65-PAK, PAK-1, Serine/threonine-protein kinase PAK 1
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PAK1/PAK2/PAK3 (Phospho-Thr423/Thr402/Thr421) Antibody #11165 (left) or the same antibody preincubated with blocking peptide (right).
Immunofluorescence staining of methanol-fixed Hela cells using PAK1/PAK2/PAK3 (Phospho-Thr423/Thr402/Thr421) Antibody #11165.
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