Anti-PER2 Antibody [ARC1134] (A307556)

Name: Anti-PER2 Antibody [ARC1134]
Brand: Antibodies.com
SKU: A307556
Price: 340.0 GBP
Availability: InStock

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$515

Rabbit monoclonal [ARC1134] antibody to PER2 for WB, ICC/IF, IP and ChIP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-PER2 Antibody [ARC1134]

Description

Rabbit monoclonal [ARC1134] antibody to PER2.

Applications

WB, ICC/IF, IP, ChIP

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200, ChIP: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PER2 (O15055).

Sequence

MNGYAEFPPSPSNPTKEPVEPQPSQVPLQEDVDMSSGSSGHETNENCSTGRDSQGSDCDDSGKELGMLVEPPDARQSPDTFSLMMAKSEHNPSTSGCSSD

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1134

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

140 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Circadian clock protein PERIOD 2, hPER2, KIAA0347, Period circadian protein homolog 2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-PER2 Antibody [ARC1134] (A307556)

Western blot analysis of extracts of various cell lines, using Anti-PER2 Antibody (A307556) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit. Exposure time: 3min.

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Immunofluorescence - Anti-PER2 Antibody [ARC1134] (A307556)

Immunofluorescence analysis of HeLa cells using Anti-PER2 Antibody (A307556) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-PER2 Antibody [ARC1134] (A307556)

Immunofluorescence analysis of NIH-3T3 cells using Anti-PER2 Antibody (A307556) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Chromatin Immunoprecipitation - Anti-PER2 Antibody [ARC1134] (A307556)

Chromatin immunoprecipitation (ChIP) analysis of extracts of MCF7 cells, using Anti-PER2 Antibody (A307556) and Rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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