Anti-Peripherin Antibody [8G2] (A85433)

Immunofluorescent analysis of human neuroblastoma cell line SH-SY5Y (A) and rat pheochromocytoma cell line PC12 (B), stained with Anti-Peripherin Antibody, at a dilution of 1:500, in red, and Anti-Vimentin Antibody (A85421 | 1:10,000, in green. The blue is DAPI staining of nuclear DNA. Peripherin, one of the Class III family of intermediate filament (IF) subunit proteins is revealed by Anti-Peripherin Antibody in the perinuclear region in some SH-SY5Y cells and in all PC12 cells. Vimentin, a protein also in the Class III IF family, is detected in a subpopulation of SH-SY5Y cells which are negative for peripherin. PC12 cells express peripherin but not vimentin.

A neuron in a rat cortical neuron culture which stains strongly for peripherin with Anti-Peripherin Antibody (red). A minority of cells in such cultures are strongly peripherin positive. In some cases they also stain for other neurofilament subunits, but this particular cell shows very little staining for NF-L using Anti-NF-L Antibody (A85451 | green). Blue is the DNA stain DAPI.

Western blot analysis of tissue and cell lysates probed with Anti-Peripherin Antibody, at a dilution of 1:500, in green,: [Lane 1] protein standard (red), [Lane 2] mouse spinal cord, [Lane 3] rat spinal cord, [Lane 4] cow spinal cord, [Lane 5] SH-SY5Y cells, and [Lane 6] PC12 cells. Band at ~57 kDa corresponds to the peripherin protein. Bands at 50 and 25 kDa, detected in the mouse spinal cord lysate are heavy and light chains of IgG.

Lane 1 shows Coomassie blue stain of a cytoskeletal extract of rat spinal cord. H, M and L indicate the positions of the three major neurofilament subunits, while G corresponds to glial fibrillary acidic protein (GFAP). Immunoblotting of Anti-Peripherin Antibody on this material is shown in Lane 2. Lane 3 shows staining with Anti-Peripherin Antibody (A85430). Peripherin runs at ~57 kDa and so is a little larger than GFAP and vimentin which run at ~50 kDa (Lane 4 shows blotting with Anti-Vimentin Antibody).