Anti-RPS6 (phospho Ser235) Antibody (A16530)

RPS6 expression in HeLa cell lysates analyzed by western blot. Cells were treated with 20% FBS at 37°C for 90 minutes following serum starvation overnight. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-RPS6 (Phospho S235) Antibody (A16530) at a dilution of 1:800 and detected with chemiluminescence.

RPS6 expression in NIH3T3 cell lysates analyzed by western blot. Cells were treated with 20% FBS at 37°C for 90 minutes following serum starvation overnight. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-RPS6 (Phospho S235) Antibody (A16530) at a dilution of 1:800 and detected with chemiluminescence.

RPS6 expression in PC-12 cell lysates analyzed by western blot. Cells were treated with Anisomycin (25 ug/ml) at 37°C for 30 minutes. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-RPS6 (Phospho S235) Antibody (A16530) at a dilution of 1:800 and detected with chemiluminescence.