This antibody targets the C-terminal domain of human SST3, detecting the non-phosphorylated isoform. It is validated for Western blot analysis, supports immunoprecipitation from cell and tissue lysates, and is suitable for immunohistochemistry in cultured cells and tissue sections. It is validated using siRNA-mediated knockdown (KO-Validated).
Applications
WB, ICC, IHC
Dilutions
WB: 1,000, IHC: 1:100, ICC: 1:200
Reactivity
Human
Immunogen
Synthetic peptide corresponding to human SST3 (amino acids. 344-364). Immunogen range is 22-22 amino acids.
Sequence
QEATPPAHRRAAANGLMQTSKL
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in Dulbecco's PBS, pH 7.4, with 150 mM NaCl and 0.005% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Somatostatin receptor type 5, SS-5-R, SS5-R, SS5R, SST5
Western blot validation of Somatostatin Receptor 5 (SST5) in transfected HEK293 cells. Lysates from native HEK293 cells (MOCK) or cells stably expressing SST5 were immunoblotted with phosphorylation-independent anti-SST5 antibody (A334547) at 1:1000.
Western blot analysis of Somatostatin Receptor 5 (SST5) in human neuroendocrine tumors. Homogenates from neuroendocrine tumor samples (T1, T2) were immunoblotted with anti-SST5 antibody (A334547) at 1:1000.
Immunocytochemical detection of Somatostatin Receptor 5 (SST5) in transfected HEK293 cells. Native HEK293 cells (MOCK) or cells stably expressing SST5 were stained with anti-SST5 antibody (A334547) at 1:200. SST5 receptors were confined to the plasma membrane in transfected cells.
Immunohistochemical localization of Somatostatin Receptor 5 (SST5) in human pancreatic islets. Pancreatic sections were dewaxed, microwaved in citric acid, and stained with anti-SST5 antibody (A334547) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed with 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. SST5 receptors were detected at the plasma membrane of nearly all pancreatic islet cells.
Detection of Somatostatin Receptor 5 (SST5) in human anterior pituitary by immunohistochemistry. Anterior pituitary sections were dewaxed, microwaved in citric acid, and incubated with anti-SST5 antibody (A334547) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed with 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. SST5 receptors were observed in a distinct population of anterior pituitary cells.
Immunohistochemical localization of Somatostatin Receptor 5 (SST5) in human growth-hormone-producing pituitary adenoma. Tumor sections were dewaxed, microwaved in citric acid, and stained with anti-SST5 antibody (A334547) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed with 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. SST5 receptors were uniformly detected at the plasma membrane of nearly all tumor cells.
Detection of Somatostatin Receptor 5 (SST5) in human pheochromocytoma by immunohistochemistry. Tumor sections were dewaxed, microwaved in citric acid, and incubated with anti-SST5 antibody (A334547) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed with 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. SST5 receptors were uniformly observed at the plasma membrane of nearly all pheochromocytoma cells.
