Anti-STAT3 (phospho Tyr705) Antibody [ARC50831] (A305396)

Western blot analysis of NIH/3T3 cells, using Anti-STAT3 (phospho Tyr705) Antibody [ARC50831] (A305396) at 1:1,000 dilution. NIH/3T3 cells were treated by TNF-alpha (20 ng/ml) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-STAT3 (phospho Tyr705) Antibody [ARC50831] (A305396) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-STAT3 (phospho Tyr705) Antibody [ARC50831] (A305396) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-STAT3 (phospho Tyr705) Antibody [ARC50831] (A305396) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.