Rabbit polyclonal antibody to STMN1 (Phospho-Ser63)
Specificity
The antibody detects endogenous levels of STMN1 only when phosphorylated at serine 63.
Applications
WB, IHC
Dilutions
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of Serine 63(R-K-S(p)-H-E) derived from Human STMN1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from COS cells treated with nocodazole using STMN1 (Phospho-Ser63) Antibody #.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STMN1 (Phospho-Ser63) antibody #11722 (left)or the same antibody preincubated with blocking peptide (right).
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