Recombinant Anti-TIM 3 Antibody [TIM3/6863R] (A315730)

Anti-TIM 3 Antibody [TIM3/6863R]

Recombinant rabbit monoclonal [TIM3/6863R] antibody to TIM 3.

TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk. TIM-3 is expressed on the surface of effector T cells (CD4+Tc2). In chronic inflammation, autoimmune disorders, and some cancers, TIM-3 is upregulated on several other hematopoietic cell types. The Ig domain of TIM-3 interacts with a ligand on resting but not activated Th1 and Th2 cells. The glycosylated Ig domain of TIM-3 binds cell-associated galectin-9. This induces TIM-3 Tyr phosphorylation and pro-apoptotic signaling. TIM-3 functions as a negative regulator of Th1 cell activity. Its blockade results in increased IFN-gamma production, Th1 cell proliferation and cytotoxicity, regulatory T cell development, and increases in macrophage and neutrophil infiltration into sites of inflammation.

PC3, BT474, HepG2, HDLM-2 or Daudi cells. Tonsil, lymph node or spleen.

IHC: 1-2 µg/ml

Human

Recombinant fragment of human TIM3 protein (around Amino Acids 1-200). The exact sequence is proprietary.

Rabbit

Monoclonal

TIM3/6863R

kappa

Protein A/G chromatography.

200 µg/ml

60 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

CD366, HAVcr-2, HAVCR2, Hepatitis A virus cellular receptor 2, T-cell immunoglobulin and mucin domain-containing protein 3, T-cell immunoglobulin mucin receptor 3, T-cell membrane protein 3, TIM-3, TIM3, TIMD-3, TIMD3