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Anti-TNF alpha Antibody [ARC52531] (A309117)

Western Blot - Anti-TNF alpha Antibody (A309117) - Antibodies.com
$515
Product Datasheet

Rabbit monoclonal [ARC52531] antibody to TNF alpha for WB.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-TNF alpha Antibody [ARC52531]
Description
Rabbit monoclonal [ARC52531] antibody to TNF alpha.
Applications
WB
Dilutions
WB: 1:500-1:1,000
Reactivity
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 77-233 of human TNF-a (NP_000585.2).
Sequence
VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC52531
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
18 kDa / 25 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Cachectin, TNF, TNF-a, TNF-alpha, TNFA, TNFSF2, Tumor necrosis factor, Tumor necrosis factor ligand superfamily member 2
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Western Blot - Anti-TNF alpha Antibody (A309117) - Antibodies.comEnlarge Image

Western Blot - Anti-TNF alpha Antibody [ARC52531] (A309117)

Western blot analysis of various lysates, using Anti-TNF alpha Antibody (A309117) at 1:1,000 dilution. THP-1 cells were treated by LPS (1 µg/ml) at 37°C for 8 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Publishing research using Anti-TNF alpha Antibody [ARC52531] (A309117)? Please let us know so that we can list the citation on this page.

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Proteins predicted to interact with TNF alpha

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score