Mouse monoclonal [J2D10] antibody to TNF alpha (CF®488A).
Applications
IHC, IHC-P, ELISA
Positive Control
HeLa, HL-60, and A431 cellsor macrophages in lymph node and tonsil tissue.
Dilutions
Flow Cytometry: 0.5-1 µg/million cells in 100 µL, IF: 1-2 µg/mL. Directly conjugated primary antibodies may require higher working concentrations than indirect detection systems.
Reactivity
Human
Cross Reactivity
No cross-reactivity with Mouse. Cross-reactivity with other species has not been determined.
Immunogen
Recombinant human TNF alpha protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
J2D10
Isotype
IgG1
Light Chains
kappa
Conjugate
CF®488A
Concentration
0.1 mg/mL
Predicted MW
17 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.1% BSA and 0.05% Sodium Azide.
Storage
Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.
