Recombinant Anti-TNF alpha Antibody [TNF/1500R] (CF®647) (A353338)

Anti-TNF alpha Antibody [TNF/1500R] (CF®647)

Recombinant rabbit monoclonal [TNF/1500R] antibody to TNF alpha (CF®647).

IHC, IHC-P

HeLa, HL-60, and A431 cells or pancreas or histiocytoma tissue.

Flow Cytometry: 0.5-1 µg/million cells in 100 µL, IF: 1-2 µg/mL, IHC-P: 4-8 µg/mL. For IHC-P, heat-induced epitope retrieval is recommended using 10 mM citrate buffer, pH 6.0, for 10-20 minutes, followed by cooling to room temperature for 20 minutes. Directly conjugated primary antibodies may require higher working concentrations than indirect detection systems.

Human, Rat

Recombinant full-length human TNF alpha protein.

Rabbit

Monoclonal

IgG

kappa

0.1 mg/mL

17 kDa

Liquid

Supplied in Phosphate Buffered Saline with 0.1% BSA and 0.05% Sodium Azide.

Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.

Cachectin, TNF, TNF-a, TNF-alpha, TNFA, TNFSF2, Tumor necrosis factor, Tumor necrosis factor ligand superfamily member 2

This product is for research use only. It is not intended for diagnostic or therapeutic use.