Human recombinant TRAP1 (amino acids 1-59 removed - transit peptide).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Protein A purification.
Concentration
1 mg/ml
Molecular Weight
~75 kDa (multiple isoforms)
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Heat shock protein 75 kDa, mitochondrial, Heat shock protein family C member 5, HSP 75, HSP75, HSPC5, TNFR-associated protein 1, TRAP-1, Tumor necrosis factor type 1 receptor-associated protein
Western blot analysis of human Cervical cancer cell line (HeLa) lysate showing detection of ~75 kDa TRAP1 protein using Anti-TRAP1 Antibody (A305020) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: human Cervical cancer cell line (HeLa) lysate. Load: 12 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 5 minutes at room temperature. Predicted/Observed Size: ~75 kDa. Other Band(s): 100 kDa.
Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-TRAP1 Antibody (A305020), at 1:120 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Mitochondrion. Mitochondrion inner membrane. Mitochondrion matrix. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-TRAP1 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-TRAP1 Antibody (A305020), at 1:120 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Mitochondrion. Mitochondrion inner membrane. Mitochondrion matrix. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-TRAP1 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
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