Purified recombinant full-length human TRAP1 protein (without tags).
Host
Mouse
Clonality
Monoclonal
Clone ID
Trap1-6
Isotype
IgG2a
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein G purification.
Concentration
1 mg/ml
Molecular Weight
~75 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Heat shock protein 75 kDa, mitochondrial, Heat shock protein family C member 5, HSP 75, HSP75, HSPC5, TNFR-associated protein 1, TRAP-1, Tumor necrosis factor type 1 receptor-associated protein
Western Blot - Anti-TRAP1 Antibody [Trap1-6] (A304865)
Western blot analysis of human Heat Shocked cervical cancer cell line (HeLa) lysate showing detection of Trap1 protein using Anti-TRAP1 Antibody [Trap1-6] (A304865) at 1:1,000 for 2 hours at room temperature. Load: 15 µg. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Sheep Anti-Mouse IgG: HRP for 1 hour at room temperature.
Immunocytochemistry/Immunofluorescence analysis of human PC3M cells, using Anti-TRAP1 Antibody [Trap1-6] (A304865), at 1:1,000. The secondary antibody used was FITC Goat Anti-Mouse (green). Counterstain: Mitotracker (red) mitochondria stain. DAPI (blue) nuclear stain.(A) staining of mitochondria with MitoTracker dye; (B) staining of nuclei with DAPI; (C) staining with TRAP1 antibody; (D) tricolor composite of images A-C..
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