Anti-Vimentin Antibody [ARC0086] (A305554)

Western blot analysis of extracts of various cell lines, using Anti-Vimentin Antibody [ARC0086] (A305554) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

Western blot analysis of extracts from wild type (WT) and Vimentin knockout (KO) 293T cells, using Anti-Vimentin Antibody [ARC0086] (A305554) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

Immunohistochemistry analysis of paraffin-embedded human liver using Anti-Vimentin Antibody [ARC0086] (A305554) at a dilution of 1:50(40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Vimentin Antibody [ARC0086] (A305554) at a dilution of 1:50(40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.