Anti-ZAP70 Antibody [ZAP70/2046] - BSA and Azide free
Description
Mouse monoclonal [ZAP70/2046] antibody to ZAP70.
Specificity
ZAP70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells.Control of this protein translation is via the IgVH gene. ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well.Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.
Applications
ELISA, IHC-P
Dilutions
IHC-P: 1-2 µg/ml
Reactivity
Human
Immunogen
Recombinant fragment, around amino acids 247-382, of human ZAP70 protein. The exact sequence is proprietary.
Host
Mouse
Clonality
Monoclonal
Clone ID
ZAP70/2046
Isotype
IgG2a
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A/G chromatography.
Concentration
1 mg/ml
Molecular Weight
70 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
SDS-PAGE analysis of Anti-ZAP70 Antibody [ZAP70/2046] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.
Anti-ZAP70 Antibody [ZAP70/2046] - BSA and Azide free (A253525)
Analysis of protein array containing more than 19,000 full-length human proteins using Anti-ZAP70 Antibody [ZAP70/2046]. Z-Score and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target; a MAb is considered to be specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Publishing research using Anti-ZAP70 Antibody [ZAP70/2046] - BSA and Azide free (A253525)? Please let us know so that we can list the citation on this page.
Alternative products to Anti-ZAP70 Antibody [ZAP70/2046] - BSA and Azide free (A253525)