Reconstitute with 0.5 ml of distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution. Further dilutions should be made in a buffer containing a carrier protein.
Endotoxin Level
< 1.1 EU/µg
Storage
Lyophilized: Store at 4°C. Reconstituted: Store at -70°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Canine tumor necrosis factor alpha (TNF alpha) is produced by a range of cells, including macrophages and monocytes and is an important mediator of the canine inflammatory response. Recombinant dog TNF alpha has an ED50 of 10 pg/ml, determined by measuring the cytolytic effect of recombinant canine TNF alpha on murine WEHI-13VAR-164 cells in the presence of actinomycin D.
