Immobilized Recombinant Human PD-L1 (10 µg/mL) bind Recombinant Human PD-1 in a functional ELISA with a linear range of 0.25-1.02 µg/mL.Immobilized PE anti-human CD274 (B7-H1, PD-L1) Antibody (1µg/mL (25 µL/well) can bind Human CD274 in a functional ELISA with a linear range of 0.46-18ng/mL.
Purity
>97% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Endotoxin Level
<0.1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Standard Curve - Recombinant Human PD-L1 Protein (C-terminal His Tag) (A331157)
ELISA examination of immobilized Human PD-L1 (200ng/mL) binding Recombinant Human PD-L1 Protein (C-terminal His Tag) (A331157): linear range = 0.06-1.25 ng/mL.
Standard Curve - Recombinant Human PD-L1 Protein (C-terminal His Tag) (A331157)
ELISA examination of immobilized Recombinant Human PD-L1 His Tag (5ug/mL) binding Recombinant Human PD-L1 Protein (C-terminal His Tag) (A331157): linear range = 0.16-1.09 µg/mL.
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