Recombinant Human PD-L1 Protein (C-terminal Mouse Fc Tag)
Expression System
HEK293 cells
Nature
Recombinant
Protein Species
Human
Tag
C-terminal Mouse Fc Tag
Conjugate
Unconjugated
Biological Activity
Immobilized Human PD-L1 (0.2 µg/mL) binds Anti-PD-L1 Ab in an ELISA: EC50 = 1.46 ng/mL.
Purity
>95% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.2µm filter sterilized).
Endotoxin Level
<0.1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Recombinant Human PD-L1 Protein (C-terminal Mouse Fc Tag) (A331155)
Tris-Bis PAGE of Recombinant Human PD-L1 Protein (C-terminal Mouse Fc Tag) (A331155) under reducing conditions confirms that purity is greater than 95%.
Standard Curve - Recombinant Human PD-L1 Protein (C-terminal Mouse Fc Tag) (A331155)
ELISA examination of immobilized Human PD-L1, mFc Tag, (0.5µg/mL) binding Recombinant Human PD-L1 Protein (C-terminal Mouse Fc Tag) (A331155): EC50 =0.9µg/ml.
Standard Curve - Recombinant Human PD-L1 Protein (C-terminal Mouse Fc Tag) (A331155)
ELISA examination of immobilized Human PD-L1, mFc Tag, (2µg/mL) binding Recombinant Human PD-L1 Protein (C-terminal Mouse Fc Tag) (A331155): EC50 =0.13µg/ml.
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