Immobilized Recombinant Human PD-1 (5 µg/mL) bind Recombinant Human PD-L1 in a functional ELISA with a linear range of 0.5-2.2 µg/mL.
Purity
>95% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Reconstitution
Centrifuge the vial before opening and reconstitute with distilled sterile water to a concentration of 0.1-0.5 mg/mL. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer.
Endotoxin Level
<0.1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Standard Curve - Recombinant Human PD-L1 Protein (C-terminal Human Fc and His Tag) (A331156)
ELISA examination of immobilized Recombinant Human PD-1 (5 µg/mL) binding Recombinant Human PD-L1 Protein (C-terminal Human Fc and His Tag) (A331156): linear range = 0.5-2.2 µg/mL.
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