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Donkey Anti-Sheep IgG H&L Antibody (CF®350), Cross-Adsorbed (A343561)

CF®350 - Donkey Anti-Sheep IgG H&L Antibody (CF®350), Cross-Adsorbed (A343561) -Antibodies.com CF®350 - Donkey Anti-Sheep IgG H&L Antibody (CF®350), Cross-Adsorbed (A343561) -Antibodies.com CF®350 - Donkey Anti-Sheep IgG H&L Antibody (CF®350), Cross-Adsorbed (A343561) -Antibodies.com CF®350 - Donkey Anti-Sheep IgG H&L Antibody (CF®350), Cross-Adsorbed (A343561) -Antibodies.com CF®350 - Donkey Anti-Sheep IgG H&L Antibody (CF®350), Cross-Adsorbed (A343561) -Antibodies.com
$195
Product Datasheet

Donkey Anti-Sheep IgG H&L (CF®350) secondary antibody for ICC/IF, IHC, WB and Flow Cytometry.

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Alternative Formats and ConjugatesAlternative Formats

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 6-9 business days.

  • Specifications

Name
Donkey Anti-Sheep IgG H&L Antibody (CF®350), Cross-Adsorbed
Description
Donkey Anti-Sheep IgG H&L (CF®350) secondary antibody.
Host Species
Donkey
Target Species
Sheep
Specificity
This product recognizes the heavy and light chains of Sheep IgG.
Applications
ICC/IF, IHC, WB, Flow Cytometry
Recommended Dilutions
IF: 1-10 µg/mL, WB: 50-100 ng/mL, IHC: 1-10 µg/mL
Clonality
Polyclonal
Isotype
IgG
Conjugate

CF®350

Product Form
Supplied as a liquid; 1 mg vials are lyophilized.
Concentration
2 mg/mL
Formulation
Liquid: Supplied in phosphate buffered saline (PBS) containing 50% glycerol, 2 mg/ml BSA, and 0.05% sodium azide
Storage
Shipped at +4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. CF® Dyes are guaranteed for at least 6 months from data of receipt when stored correctly.
General Notes

The blue dye conjugate CF®350 is not recommended for the detection of low-abundance targets, as blue fluorophores typically produce lower fluorescence intensity and may result in increased non-specific background compared with dyes of other spectral regions.

Looking for a specific protein conjugate to simplify your workflow? We offer a library of over 2,000 targets conjugated to your choice of CF® dye. To enquire about a custom product, contact us directly.

Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (5)

CF®350 - Donkey Anti-Sheep IgG H&L Antibody (CF®350), Cross-Adsorbed (A343561) -Antibodies.comEnlarge Image

Donkey Anti-Sheep IgG H&L Antibody (CF®350), Cross-Adsorbed (A343561)

Relative fluorescence intensity of CF®350 and Alexa Fluor® 350 goat anti-mouse antibody conjugates plotted as a function of degree of labeling.
CF®350 - Donkey Anti-Sheep IgG H&L Antibody (CF®350), Cross-Adsorbed (A343561) -Antibodies.comEnlarge Image

Donkey Anti-Sheep IgG H&L Antibody (CF®350), Cross-Adsorbed (A343561)

HeLa cells immunostained with mouse anti-tubulin primary antibody and CF®350 goat anti-mouse secondary antibody.
CF®350 - Donkey Anti-Sheep IgG H&L Antibody (CF®350), Cross-Adsorbed (A343561) -Antibodies.comEnlarge Image

Donkey Anti-Sheep IgG H&L Antibody (CF®350), Cross-Adsorbed (A343561)

Normalized spectra of CF®350 goat anti-mouse conjugate in PBS.
CF®350 - Donkey Anti-Sheep IgG H&L Antibody (CF®350), Cross-Adsorbed (A343561) -Antibodies.comEnlarge Image

Donkey Anti-Sheep IgG H&L Antibody (CF®350), Cross-Adsorbed (A343561)

This chart summarizes commonly used CF® dyes grouped by their suitability for specific imaging modalities, including alternative spectra applications, four-color confocal imaging, near-infrared western blotting, photoacoustic imaging, STED, SIM, STORM, TIRF, and two-photon microscopy. Each dye is shown with its characteristic excitation and emission wavelengths (nm), providing a practical reference for selecting spectrally compatible dyes and optimizing multicolor experimental design across a range of fluorescence techniques.
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