This antibody recognises the murine CD34 cell surface antigen, expressed by endothelial cells and by hematopoietic stem cells. recognises a neuraminidase sensitive epitope. As in the human system, CD34 antibodies in the mouse demonstrate slightly different staining patterns depending on their fine specificity. Rat anti Mouse CD34 antibody, clone MEC14.7 appears to recognize a subset of the stem cell population recognized by clone RAM34, and it is thought that this is due to differences in the epitope recognized by the two antibodies.
Applications
IHC-P, IP, Flow Cytometry
Dilutions
Flow Cytometry: 1:10 for 0.25 mg, 25 µg, 1:25 for 0.1 mg - 1:20 for 0.25 mg, 25 µg, 1:50 for 0.1 mg, IHC-P: 1:20 - 1:200, IP: 2 µg/ml - 10 µg/ml, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Mouse
Immunogen
T-end.1, a pMT transformed endothelial cell line.
Host
Rat
Clonality
Monoclonal
Clone ID
MEC14.7
Isotype
IgG2a
Conjugate
Unconjugated
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Rat anti Mouse CD34 antibody, clone MEC14.7 recognizes the murine CD34 cell surface antigen, expressed by endothelial cells and by hematopoietic stem cells. Rat anti Mouse CD34 antibody, clone MEC14.7 recognizes a neuraminidase sensitive epitope. As in the human system, CD34 antibodies in the mouse demonstrate slightly different staining patterns depending on their fine specificity. Rat anti Mouse CD34 antibody, clone MEC14.7 appears to recognize a subset of the stem cell population recognized by clone RAM34, and it is thought that this is due to differences in the epitope recognized by the two antibodies.
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