Anti-CD45 Antibody [OX-29] (A281432)

Anti-CD45 Antibody [OX-29]

Mouse monoclonal [OX-29] antibody to CD45.

This antibody recognises rat CD45, also known as Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen (LCA) or T200, expressed on all rat leukocytes. CD45 is a single pass, type I transmembrane glycoprotein of ~180-240kDa depending on the isoform and degree of glycosylation. Rat CD45 contains two intracellular tyrosine-protein phosphatase domains and at least two extracellular fibronectin type-III domains (Uniprot Acc no: P04157), although the existence of a third similar domain has previously been proposed. Mouse anti Rat CD45 antibody, clone OX-29 has been reported as recognizing a monomorphic determinant present on all allogenic forms of the rat CD45 antigen. Epitope mapping further indicates that the OX-29 clone binds to a determinant located within the membrane distal two domains of CD45 (Symons, et al. 1999).

IP, Flow Cytometry

Flow Cytometry: 1:10 - 1:50, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Rat

L-CA (CD45).

Mouse

Monoclonal

OX-29

IgG2a

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Rat CD45 antibody, clone OX-29 recognizes rat CD45, also known as Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen (LCA) or T200, expressed on all rat leukocytes. CD45 is a single pass, type I transmembrane glycoprotein of ~180-240kDa depending on the isoform and degree of glycosylation. Rat CD45 contains two intracellular tyrosine-protein phosphatase domains and at least two extracellular fibronectin type-III domains (Uniprot Acc no: P04157), although the existence of a third similar domain has previously been proposed (Symons et al. 1999).Mouse anti Rat CD45 antibody, clone OX-29 has been reported as recognizing a monomorphic determinant present on all allogenic forms of the rat CD45 antigen (Symons and Barclay 2000). Epitope mapping further indicates that the OX-29 clone binds to a determinant located within the membrane distal two domains of CD45 (Symons, et al. 1999).

L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200