This antibody recognises the rat CD5 cell surface antigen, a 69kD glycoprotein expressed by T cells, thymocytes and a subset of B cells. Mouse anti Rat CD5 antibody, clone OX-19 has been reported as being suitable for use on periodate-lysine paraformaldehyde (PLP) fixed paraffin embedded tissue. Mouse anti Rat CD5 antibody, clone OX-19 is routinely tested in flow cytometry on rat splenocytes.
Applications
IHC-P, IHC-Fr, Flow Cytometry, IP
Dilutions
Flow Cytometry: 1:25 - 1:100, Use 10µl of the suggested working dilution to label 106 cells in 100µl.
Reactivity
Rat
Immunogen
Rat Thymocyte glycoproteins.
Host
Mouse
Clonality
Monoclonal
Clone ID
OX-19
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Rat CD5 antibody, clone OX-19 recognizes the rat CD5 cell surface antigen, a 69kD glycoprotein expressed by T cells, thymocytes and a subset of B cells. Mouse anti Rat CD5 antibody, clone OX-19 has been reported as being suitable for use on periodate-lysine paraformaldehyde (PLP) fixed paraffin embedded tissue (Whiteland et al. 1995). Mouse anti Rat CD5 antibody, clone OX-19 is routinely tested in flow cytometry on rat splenocytes.
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