Anti-CDC25B (phospho Ser323) Antibody (A93682)

Name: Anti-CDC25B (phospho Ser323) Antibody
Brand: Antibodies.com
SKU: A93682
Price: 185.0 USD
Availability: InStock

This product is identical to Abcam (ab53103). Discover more for less.

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$185

Rabbit polyclonal antibody to CDC25B (phospho Ser323) for WB and ELISA.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-CDC25B (phospho Ser323) Antibody

Description

Rabbit polyclonal antibody to CDC25B (phospho Ser323).

Specificity

This antibody detects endogenous levels of CDC25B only when phosphorylated at Ser323.

Applications

WB, ELISA

Dilutions

WB: 1:500-1:1000, IHC: 1:50-1:100, ELISA: 1:40000

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide derived from human CDC25B around the phosphorylation site of Ser323 (amino acids 289-338).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.

Molecular Weight

64kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AI604853, Cdc 25B, CDC25HU2, Cdc25m2, Cell division cycle 25 homolog B, Cell division cycle 25B, Cell division cycle 25B isoform 1, Cell division cycle 25B isoform 2, Cell division cycle 25B isoform 3, Cell division cycle 25B isoform 4, Cell division cycle 25B isoform 5, Dual specificity phosphatase Cdc25B, EC 3.1.3.48, M phase inducer phosphatase 2, M-phase inducer phosphatase 2, MPIP2_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-CDC25B (phospho Ser323) Antibody (A93682)

Western blot analysis of lysates from NIH/3T3 cells treated with PMA 125ng/ml 30' using Anti-CDC25B (phospho Ser323) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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Figure 2: Western Blot - Anti-CDC25B (phospho Ser323) Antibody (A93682)

Western blot analysis of various cells using Anti-CDC25B (phospho Ser323) Antibody.

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Figure 3: Western Blot - Anti-CDC25B (phospho Ser323) Antibody (A93682)

Western blot analysis of VEC cells using Anti-CDC25B (phospho Ser323) Antibody.

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Figure 4: Immunohistochemistry - Anti-CDC25B (phospho Ser323) Antibody (A93682)

Immunohistochemical analysis of paraffin-embedded human breast cancer using Anti-CDC25B (phospho Ser323) Antibody 1:100 (4°C overnight). The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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