Rabbit polyclonal antibody to BRCA1 (Phospho-Ser1423)
Specificity
The antibody detects endogenous level of BRCA1 only when phosphorylated at serine 1423.
Applications
WB, IHC
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of serine 1423 (H-G-S(p)-Q-P) derived from Human BRCA1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Breast cancer type 1 susceptibility protein, RING finger protein 53, RING-type E3 ubiquitin transferase BRCA1, RNF53
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BRCA1 (Phospho-Ser1423) Antibody #11242 (left) or the same antibody preincubated with blocking peptide (right).
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