This antibody reacts with extracellular (N-terminal) domain of standard CD44 (Phagocyte glycoprotein 1), a 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes.
Applications
Flow Cytometry, IP, WB, IHC-P
Dilutions
Flow Cytometry: 4 µg/ml, WB: 2 µg/ml, 60 min on vertical incubator; Positive Control: Kg-1a human acute leukemia cell lysate, JURKAT human leukemia T-cell lysate, Non-reducing conditions, 6% separating gel, IHC-P: 10 µg/ml; Positive Control: uterus, myometrium.
Reactivity
Porcine, Human, Canine
Immunogen
COS-7 (African Green Monkey) cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-263
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
80-95 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using Anti-CD44 Antibody [MEM-263] (A85675). Lane 1: lysate of human PBLLane 2: lysate of canine PBLLane 3: lysate of porcine PBL
Separation of human CD44 positive lymphocytes (red-filled) from CD44 negative blood debris (black-dashed) in flow cytometry surface staining of human peripheral whole blood, labeling CD44 with Anti-CD44 Antibody [MEM-263] (A85675), (concentration in sample 4 µg/ml) GAM APC.
Western blotting analysis of CD44 in MOLT-4 cells (lane 1) and HeLa cells (lane 2) using Anti-CD44 Antibody [MEM-263] (A85675) purified; non-reducing conditions.
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