Mouse monoclonal [341] antibody to CD8 beta (FITC).
Specificity
This antibody, also known as Clone 34.1, recognizes rat CD8b, the 32-34 kDa beta chain of the CD8 coreceptor (extracellular epitope), expressed on T cell subsets and some other cell types, such as macrophages.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: 1-4 µg/ml
Reactivity
Rat
Immunogen
CD8 positive Wistar rat splenic T cell hybridomas.
Host
Mouse
Clonality
Monoclonal
Clone ID
341
Isotype
IgG1
Light Chains
kappa
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Purification
This antibody is conjugated with Fluorescein Isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Concentration
500 µg/ml
Predicted MW
32-34 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Separation of rat CD8b positive cells (red-filled) from CD8b negative cells (black-dashed) in flow cytometry analysis (surface staining) rat splenocyte suspension stained with Anti-CD8 beta Antibody [341] (FITC) (A86241), (concentration in sample 1 µg/ml).
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