Rabbit polyclonal antibody to FKHR (Phospho-Ser319)
Specificity
The antibody detects endogenous level of FKHR only when phosphorylated at serine 319.
Applications
WB, IHC, ICC/IF
Dilutions
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100 Immunofluorescence: 1:100~1:200
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of serine 319 (T-S-S(p)-N-A) derived from Human FKHR / FOXO1A.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Predicted MW
78-82 kd
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Forkhead box protein O1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma, FOXO1A
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FKHR (Phospho-Ser319) Antibody #11136 (left) or the same antibody preincubated with blocking peptide (right).
