Rabbit polyclonal antibody to FKHRL1 (Phospho-Ser253)
Specificity
The antibody detects endogenous level of FKHRL1 only when phosphorylated at serine 253.
Applications
WB, IHC, ICC/IF
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of serine 253(A-V-S(p)-M-D) derived from Human FKHRL1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Predicted MW
97kd
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
AF6q21 protein, Forkhead box protein O3, Forkhead in rhabdomyosarcoma-like 1, FOXO3
Western blot analysis of extracts from NIH/3T3 cells using FKHRL1 (Ab-253) antibody (#21171, Lane 1 and 2) and FKHRL1 (phospho-Ser253) antibody (#11157, Lane 3 and 4).
