Rabbit polyclonal antibody to Her3 / ErbB3 (phospho-Tyr1328)
Specificity
The antibody detects endogenous level of Her3 / ErbB3 only when phosphorylated at tyrosine 1328.
Applications
WB, IHC, ICC/IF
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of tyrosine 1328 (P-D-Y(p)-W-H) derived from Human Her3 / ErbB3.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Her3/ErbB3 (Phospho-Tyr1328) Antibody #11510 (left) or the same antibody preincubated with blocking peptide (right).