The antibody detects endogenous level of total HSP27 protein.
Applications
WB, IHC
Dilutions
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100
Reactivity
Human
Immunogen
Peptide sequence around aa.76~80 (A-L-S-R-Q) derived from Human HSP27.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Concentration
1.0 mg/mL
Predicted MW
27kd
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, Heat shock protein beta-1, Heat shock protein family B member 1, HSP 27, HSP28, HspB1, SRP27, Stress-responsive protein 27
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27 (Ab-78) Antibody #21239 (left) or the same antibody preincubated with blocking peptide (right).
Publishing research using Anti-HSP27 (Ab-78) Antibody (A36487)? Please let us know so that we can list the citation on this page.
Alternative products to Anti-HSP27 (Ab-78) Antibody (A36487)