Rabbit polyclonal antibody to HSP27 (Phospho-Ser82)
Specificity
The antibody detects endogenous level of HSP27 only when phosphorylated at serine 82.
Applications
WB, IHC
Dilutions
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of serine 82 (Q-L-S(p)-S-G) derived from Human HSP27.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Predicted MW
27kd
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, Heat shock protein beta-1, Heat shock protein family B member 1, HSP 27, HSP28, HspB1, SRP27, Stress-responsive protein 27
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27 (Phospho-Ser82) Antibody #11248 (left) or the same antibody preincubated with blocking peptide (right).
![Immunohistochemistry - Anti-HSP27 Antibody [G3.1] (A248872) - Antibodies.com](https://cdn.antibodies.com/image/catalog/248/A248872_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-HSP27 Antibody [8A7] (A304709) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304709_1.png?profile=product_alternative)
![Immunohistochemistry - Anti-HSP27 Antibody [G3.1] - BSA and Azide free (A252052) - Antibodies.com](https://cdn.antibodies.com/image/catalog/252/A252052_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-HSP27 Antibody [SPM252] (A248873) - Antibodies.com](https://cdn.antibodies.com/image/catalog/248/A248873_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-HSP27 Antibody [SPM252] - BSA and Azide free (A252053) - Antibodies.com](https://cdn.antibodies.com/image/catalog/252/A252053_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-HSP27 Antibody [HSPB1/774] (A248874) - Antibodies.com](https://cdn.antibodies.com/image/catalog/248/A248874_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-HSP27 Antibody [HSPB1/774] - BSA and Azide free (A252054) - Antibodies.com](https://cdn.antibodies.com/image/catalog/252/A252054_1.jpg?profile=product_alternative)
![SDS-PAGE - Anti-HSP27 Antibody [CPTC-HSPB1-2] - BSA and Azide free (A252055) - Antibodies.com](https://cdn.antibodies.com/image/catalog/252/A252055_1.jpg?profile=product_alternative)
![SDS-PAGE - Anti-HSP27 Antibody [CPTC-HSPB1-2] (A248875) - Antibodies.com](https://cdn.antibodies.com/image/catalog/248/A248875_1.jpg?profile=product_alternative)