Anti-MeCP2 Antibody (A87676)

Name: Anti-MeCP2 Antibody
Brand: Antibodies.com
SKU: A87676

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This product is discontinued

Anti-MeCP2 Antibody (A87676) has been discontinued and is no longer available.

View all MeCP2 Antibodies.

Specifications

Name

Anti-MeCP2 Antibody

Description

Rabbit polyclonal antibody to MeCP2.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:100

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human MECP2 (NP_004983.1).

Sequence

MVAGMLGLREEKSEDQDLQGLKDKPLKFKKVKKDKKEEKEGKHEPVQPSAHHSAEPAEAGKAETSEGSGSAPAVPEASASPKQRRSIIRDRGPMYDDPTLPEGWTRKLKQRKSGRSAGKYDVYLINPQGKAFRSKVELIAYFEKVGDTSLDPNDFDFTVTGRGSPSRREQKPPKKPKSPKAPGTGRGRGRPKGSGTTRPKAATSEGVQVKRVLEKSPGKLLVKMPFQTSPGGKAEGGGATTSTQVMVIKRPGRKRKAEADPQAIPKKRGRKPGSVVAAAA

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

60 kDa / 80 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

MeCp-2 protein, Methyl-CpG-binding protein 2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-MeCP2 Antibody (A87676)

Western blot analysis of extracts of various cell lines, using Anti-MeCP2 Antibody (A87676) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Western Blot - Anti-MeCP2 Antibody (A87676)

Western blot analysis of extracts from normal (control) and MECP2 Rabbit polyclonal antibody knockout (KO) 293T cells, using Anti-MeCP2 Antibody (A87676) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Immunohistochemistry - Anti-MeCP2 Antibody (A87676)

Immunohistochemistry analysis of paraffin-embedded human gastric cancer using Anti-MeCP2 Antibody (A87676) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-MeCP2 Antibody (A87676)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-MeCP2 Antibody (A87676) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.